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9. | | ZIOBER, I. L.; PAIÃO, F. G.; BINNECK, E.; COUTINHO, L. L.; NEPOMUCENO, A. L.; SHIMOKOMAKI, M. Identificação de diferentes transcritos do gene que codifica proteína receptora de rianodina tipo 1 em frangos submetidos ao teste do halotano. In: SEMANA DE BIOTECNOLOGIA, 4., 208, Londrina. Anais... Londrina: UEL. Departamento de Bioquímica e Biotecnologia, 2008. p. 22. Biblioteca(s): Embrapa Soja. |
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10. | | VITORINO, J. C.; SILLA, P. R.; CAMARGO-BRUNETTO, M. A. de.; BINNECK, E. Abordagem computacional para a identificação de elementos cis-regulatórios no genoma da soja. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 5., 2010, Londrina. Resumos... Londrina: Embrapa Soja, 2010. p. 95-97. (Embrapa Soja. Documentos, 323). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo. Biblioteca(s): Embrapa Soja. |
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11. | | VITORINO, J. C.; SILLA, P. R.; CAMARGO-BRUNETTO, M. A. de.; BINNECK, E. Abordagem computacional para a identificação de elementos cis-regulatóriso no genoma da soja. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 5., 2010, Londrina. Resumos... Londrina: Embrapa Soja, 2010. p. 95-97. (Embrapa Soja. Documentos, 323). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo. Biblioteca(s): Embrapa Soja. |
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15. | | SOUZA, T. D.; GODOY, S. M.; FELICIANO, D. C.; BINNECK, E.; SÓSA-GOMEZ, D. R. Genetic diversity of the entomopathogenic fungus metarhizium rileyi based on SSR. In: INTERNATIONAL CONGRESS ON INVERTEBRATE PATHOLOGY AND MICROBIAL CONTROL, 2023; ANNUAL MEETING OF THE SOCIETY FOR INVERTEBRATE PATHOLOGY, 55., 2023, Maryland. Program and abstracts... [Verona]: Society for Invertebrate Pathology, 2023. PF-4. p. 80. Biblioteca(s): Embrapa Soja. |
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17. | | COSTAMILAN, L. M.; ALMEIDA, A. M. R.; YORINORI, J. T.; SEIXAS, C. D. S.; BINNECK, E. Diaporthe phaseolorum var. caulivora, primeira ocorrência deste fungo associado ao cancro da haste da soja, no Brasil. Fitopatologia Brasileira, Brasília, DF, v. 32, p. S146, ago. 2007. Suplemento, ref. 0180. Edição dos Resumos do XL Congresso Brasileiro de Fitopatologia, Maringá, ago. 2007. Biblioteca(s): Embrapa Soja; Embrapa Trigo. |
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18. | | TURCHETTO, C.; GONZÁLES, H. H. S.; TORRES, G. A. M.; NHANI, A.; CONSOLI, L.; BINNECK, E. Suppression subtractive hybridization analysis of genes regulated by Magnaporthe oryzae infection in wheat adult plants. In: CONGRESSO BRASILEIRO DE GENÉTICA, 61., 2015, Águas de Lindóia. Pós-genômica: [resumos]. [Ribeirão Preto]: Sociedade Brasileira de Genética, [2015]. p. 34. Biblioteca(s): Embrapa Trigo. |
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Registros recuperados : 176 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
17/02/2009 |
Data da última atualização: |
17/02/2009 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SOSA-GÓMEZ, D. R.; BINNECK, E.; MARIN, S. R. R. |
Afiliação: |
Daniel Ricardo Sosa-Gómez, CNPSo; Eliseu Binneck, CNPSo; Silvana Regina Rockenbach Marin, CNPSo. |
Título: |
Nuevo método de PCR para estudiar Ia región espaciadora intergénica de Nomuraea rileyi (Farlow) Samson. New PCR method to study the intergenic spacer (IGS) region of Nomuraea rileyi (Farlow) Samson. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: CONGRESO LATINOAMERICANO DE MICOLOGIA, 6., 2008, Mar del Plata. Libro de resúmenes. Buenos Aires: Asociación Latinoamericana de Micologia, 2008. p. III. Anexo. |
Idioma: |
Espanhol |
Conteúdo: |
The variability in the IGS region of the rDNA gene complex has proven useful in the development of PCR primers for strain identification. However, the lack of information on this region in Nomuraea rileyi has precluded the development of specific primers for PCR amplification. Also, due to the high variability in this region, amplification is not possibIe with universal primers. Thus, to obtain sequences of the of the region a new approach was used, a combination of IGS primer with 10-mer oligonucleotides (OD11 or EO4, Operon Technologies) were used. The amplification products were visualized by agarose gel electrophoresis. DNA bands were selected for comparison between IGS-10 mer primer product amplification and DNA bands produced by 10-mer amplification. When DNA products were absent in the RAPD amplification and present in the combination IGS-10 mer amplification, these products were gelpurified, ligated into the plasmid vector TOPO TA (lnvitrogen), and amplified in DH10 B Escherichia coli cells. The DNA obtained from positive clones were subjected to Sanger sequencing. The obtained sequences ranged from 1,027 to 1,064 bases. Comparisons among isolates obtained from the same geographic region suggest that this technique could be used to develop strain specific primers for N. rileyi.
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Categoria do assunto: |
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Marc: |
LEADER 01962naa a2200145 a 4500 001 1471173 005 2009-02-17 008 2008 bl --- 0-- u #d 100 1 $aSOSA-GÓMEZ, D. R. 245 $aNuevo método de PCR para estudiar Ia región espaciadora intergénica de Nomuraea rileyi (Farlow) Samson. New PCR method to study the intergenic spacer (IGS) region of Nomuraea rileyi (Farlow) Samson. 260 $c2008 520 $aThe variability in the IGS region of the rDNA gene complex has proven useful in the development of PCR primers for strain identification. However, the lack of information on this region in Nomuraea rileyi has precluded the development of specific primers for PCR amplification. Also, due to the high variability in this region, amplification is not possibIe with universal primers. Thus, to obtain sequences of the of the region a new approach was used, a combination of IGS primer with 10-mer oligonucleotides (OD11 or EO4, Operon Technologies) were used. The amplification products were visualized by agarose gel electrophoresis. DNA bands were selected for comparison between IGS-10 mer primer product amplification and DNA bands produced by 10-mer amplification. When DNA products were absent in the RAPD amplification and present in the combination IGS-10 mer amplification, these products were gelpurified, ligated into the plasmid vector TOPO TA (lnvitrogen), and amplified in DH10 B Escherichia coli cells. The DNA obtained from positive clones were subjected to Sanger sequencing. The obtained sequences ranged from 1,027 to 1,064 bases. Comparisons among isolates obtained from the same geographic region suggest that this technique could be used to develop strain specific primers for N. rileyi. 700 1 $aBINNECK, E. 700 1 $aMARIN, S. R. R. 773 $tIn: CONGRESO LATINOAMERICANO DE MICOLOGIA, 6., 2008, Mar del Plata. Libro de resúmenes. Buenos Aires: Asociación Latinoamericana de Micologia, 2008. p. III. Anexo.
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